I am planning on staining neuronal cells in culture to visualize neurites. Thermofisher's Vybrant-CM DiI seems the best fit for my requirements. However, I am unable to find any images or papers that show the use of the this stain on neuronal cells. I am now reconsidering the usage of this stain, and am also considering the Vybrant DiI solution or the DiI crystals also sold by Thermofisher. If you have used this with neurons, please comment on it!
NOTE: I aim to use a non-injectable stain.
Hi Kavya Kalpana Ganesh - just curious as to whether you tried the Vybrant DiI cell labeling solution or crystals? I'm also planning to stain fixed cultured neurons and wondered if you had any luck. Thanks!
Kerry Rennie Hi yes! I used the labeling solution in the end- it just fit my protocol, including other stains, well. I incubated my cells with the stain for 30 mins. I also used it with a different solvent than what's specified in the manual, but it still worked okay. However, there was a lot of fluorescent leakage to the green channel (I used one for the orange wavelength), so that's something to consider. Hope this helps!
P.S. I'd be really interested in knowing if you observe some fluorescence leak as well, let me know!
Kavya Kalpana Ganesh thanks for taking the time to answer! I'll let you know how it turns out for me.
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