Hi.
I am looking for the information about detection beta-endorphin and POMC in the rat brain. What method will be the best (ELISA, Western blot)?
Which part of the rat brain (or maybe homogenized the whole brain) should be taken to observe the changes in beta-endorphin and POMC?
André Barreto Vianna
If the POMC you refer to, is the Pro-opiomelanocortin, then the best (if not the only) place to observe changes is in the hypothalamus.
For POMC, the best method is Western Blotting and Immunohistochemistry.
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Krystyna Cegielska-Perun
Thank you very much for your answers. Have you ever isolated the rat hypothalamus? Is it difficult? And how to do it?
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Enrico Marani
Look into literature on the arcuate hypothalamic nucleus and the lateral ventral parts of the hypothalamus.
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