Does anyone have any experience with long-term storage/freezing of CFSE-stained bacterial cells and their later use in experiments?
Do you notice any difference in the fluorescence levels from the frozen CFSE-stained stock versus freshly stained bacteria?
Krystyna Liskiewicz depends on your application and if you need them to be alive or not.
If you need them to be viable, they can be stored in PBS+20% of glycerol in dark eppendorf tubes. If you don't care if they are alive or not, you can just freeze them in PBS or DPBS. If you keep them protected from light, generally there is no decrease in fluorescence.
As a general rule, I would still recommend to prepare them fresh before the experiments since defrosting might affect your assay. But again it depends on what are you trying to do.
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