I am writing to seek advice and guidance regarding an issue my laboratory is currently facing. Unfortunately, due to budget constraints, we are unable to pursue expensive methods for the detection of microsatellite instability (MSI) in gastric tumors.
We have at our disposal paraffin-embedded tissue blocks, as well as DNA and RNA extracted from the samples. Considering our limited resources, I am keen to explore the most straightforward and cost-effective method for MSI detection. If anyone could share insights or recommend a reliable approach using the available materials, it would be greatly appreciated.
Additionally, I want to provide some context. Over the past two years, I have attempted to standardize the High Resolution Melting (HRM) technique on the QIAGEN Rotor Gene Q instrument. Unfortunately, I have been unsuccessful in obtaining satisfactory results, particularly with the BAT25 and BAT26 markers. Strangely, only the control appeared microsatellite stable (MSS), while all the samples consistently exhibited microsatellite instability (MSI).
I am open to any suggestions, tips, or alternative methods that could help us overcome these challenges within our financial constraints. Thank you in advance for any assistance you can provide.
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