Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Abaqus static analysis - Applied tensile axial force on B21 cantiliver beam does not balance reaction. Can you please advise on resolving this?

I have modeled a cantilever beam in Abaqus to test metal plasticity option. The beam is cantilever, 10 m long, and discretized into 10 B21 elements with 1m x 1m cross section. The beam material is...

01 January 2021 696 0 View

Shall we split the data into 60% of training and 40% of testing. And apply k fold cross validation for training and the best trained model for test?

Is the above method of splitting the data set into taring and testing, and then apply k fold cross validation for training, and then the trained model to classify the test set is right?

04 April 2020 9,040 3 View

Scalogram for huge data?

I have a huge data for which I want to apply wavelet and plot scalogram. the below code works for small data. However, I have a huge signal, for which I am not able to plot scalogram. Can anyone...

10 April 2019 1,959 3 View

How to do miRNA isolation from hematopoietic stem cell with good purity?

Hi, currently I am isolating miRNA from hematopoietic stem cell using miRNeasy Qiagen kit. However, I didnt get good purity for both A260/280 and A260/230 plus low concentration as well. According...

14 October 2018 6,123 1 View

What are the causes for low Free Fatty Acids (0.16) in Cocoa mass?

Free fatty acids content should not be more than 1.75% in cocoa mass according to FSSAI standards and got 0.16 % as result. I want to know the reason behind occurrence of low free fatty acids in...

22 November 2017 9,993 4 View

How to concentrate DNA? I lost 70% of DNA while concentrating it using 3M sodium acetate and 2 volumes of ethanol? Is there any other alternative?

Read 7 answers by scientists to the question asked by Ramya Murugesan on Oct 6, 2016

05 October 2016 9,702 6 View

Does Lysozyme cause the lysis of cell wall as well as inner membrane in gram positive bacteria?

08 May 2016 289 14 View

Does J774A.1 form clumps in cell culture flask?

I see the newly thawed J774A.1 cells form clumps of about 10-20 cells. On sub-culturing the size of the clump reduced to about 5-10. Is that normal? I am using DMEM media supplemented with 10%FBS...

10 January 2016 2,226 5 View

Are there any books for image processing techniques using verilog?

Are there any books for image processing techniques using HDL verilog?

05 January 2016 7,344 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View