Gadicherla Ramya

4 Questions 10 Answers 0 Followers

Questions related from Gadicherla Ramya

Is there procedure for Isolation of DNA and RNA from Tissues stored in Formalin?

Hi I want to isolate DNA as well as RNA[Specifically miRNA] from Biopsy tissues stored in Formalin. I would like to know if the tissues need to be treated before proceeding for nucleic acid...

19 February 2016 3,022 3 View

Number of samples for discovery based study by NGS?

Hi all, To conduct a cancer based discovery study by NGS what is the optimal number of samples [minimum number to be precise] which would yield convincing results as it is difficult to sponsor for...

13 November 2015 6,017 3 View

How to I detect contaminant sequences in NGS data?

Hi, we have sequenced 6 bacteria of same species on an NGS platform. When we aligned the data to the reference the alignment was only 2%. When we checked for a possible contamination, the...

06 October 2015 468 8 View

How do we finish the draft genome from ngs data? Also how to call snp's from draft genomes?

Hi all, We have sequenced a bacterial genome. It is a denovo sequencing. We need to take the sequence of it and use that as a reference and call snp's from 4 more samples which belonging to same...

10 June 2015 3,863 6 View