It was mentioned ,"The experimentation consisted of batch cultures in 2-L conical glass Erlenmeyer flasks were filled with 2 L saline water" but it is not possible to have culture volume at 2L in 2L conical glass flask as they were aerating the culture medium. May be it is a printing mistake. I want to know about the exact volume of media used for culturing Phormidium as I work on filamentous cyanobacteria.
Despite the specie used, usually the working volume of an aerating reactor (air-lift) is the 70% of the total volume of the container.
I don't suggest an air-lift in conical flask, because if foam is produced this would come out of the flask more easly than a standard bottle.
-N BG 11,Because is is osscillatoriales,because -N will allow the heterocyst to appear much clearly
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