Naresh Kumar Manda

3 Questions 11 Answers 0 Followers

Questions related from Naresh Kumar Manda

What to do if protein is truncating during expression in E.coli..?

I am expressing two bacterial proteins in E.coli one is truncating and appearing 7 KDa below the actual size on sds. another protein is forming oligomer or dimer and giving extra band at higher...

18 January 2022 1,553 5 View

What is the best way of disruption of genes inside the yeast and algal cells...? create point mutations ...? or CRISPR/Cas9...?

I am working on the disruption of some yeast genes to create new strains... for my research project "Metabolic Engineering of Saccharomyces cerevisiae as lipid cell factories for Bio-Diesel...

16 October 2021 9,427 1 View

How to visualize and identify the formation of lipid droplets in saccharomyces cerevisiae.?

How to visualize and identify the formation of lipid droplets .. like wether they are micro lipid droplets or macro lipid droplets..?

02 November 2018 6,148 2 View