I want to start work with PC-12 cell line and I want to order nerve growth factor to differentiate these cells and I do not know which sort I should order. It will be so helpful if some body working with this cell line give me the fall name of NGF which they use for the differentiated?
It's been a while since I've worked with PC12 cells, but I found this protocol online which might be of use for you, and seems consistent with what I was doing before: http://www.hopkinsmedicine.org/institute_basic_biomedical_sciences/research_centers/metabolism_obesity_research/protocols/pdfs/A8b_PC.pdf
Thank you Troy for responding but do you think 10-12 days will be long time??
Hello Sarah, I use NGF 7S from Thermo Scientific and I use it for 7 days maximum.
human beta-NGF from Alomone (100 ng/mL): PC12 differentiation and neurite outgrowth in 3-4 days.
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