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Questions related from Luciana Sampieri
I have been working with PC12 cells for years. Lately, however, I noticed that they start dying after 3 days of differentiation with NGF . My protocol is the following: I plate the cells onto poly...
01 January 2019 5,101 1 View
I am using KT5720, a PKA inhibitor. The first time I used it, it had no effect at 1 uM 1 hour before my experimental treatment in PC12 cells. I would like to know what is the optimal time of...
09 September 2017 1,690 1 View
I have a new set of primers and I need to determine the best concentration for qPCR assays. How is it done?
04 April 2017 3,493 8 View
I am working with PC12 cells and would like to know what are the best conditions to perform western blot of alpha synuclein (14 kDa). Especially, regarding: best buffer for protein extraction:...
05 May 2016 8,232 1 View
What is the the best concentration of Rotenone to treat differentiated PC12? How should I make and store the stock solution? Thanks
05 May 2016 7,377 0 View
I am working with PC12 cells and I take mRNA samples every 48 hours after differentiation with NGF. Which would be the best endogenous control for qPCR given that it needs to be one that does not...
05 May 2016 4,661 1 View
I am working with PC12 cells and. My problem is that when I perform anti alpha-synuclein Western Blots, the signals of this protein are never consistent with each other, even when I run duplicates...
06 June 2015 794 5 View
I want to test effects of a toxic drug on SH-SY5Y cells and I read that there are many agents that induce differentiation into different phenotypes but I could not conclude which of them are...
04 April 2015 5,153 3 View
I am trying to perform western blots of alpha synuclein (14 kDa), using wet transference 1h 15min at 85 V, but I get this weird "patchy" pattern, the band isn't a whole defined band. What could be...
04 April 2014 1,963 15 View
