There are any one helping me to get a protocol for induction cancer in mice?
I need to do tow ways ANOVA on the last version of the graph pads and I could not find the real value of p. it is always adjusted value
05 June 2018 6,177 0 View
Dear All, I have 2 excel sheets, one of them to 100 treatment group with 10 parameters. The another sheet with control group with same parameters( 10 parameters). My question is how I can add the...
03 April 2018 2,053 5 View
Hello every body, I would like to measure level of Catalase activity in PC12 cells after lead exposure. I used phosphate buffer and H2O2 method to measure that. I have not get any deception in...
10 November 2015 7,257 3 View
I am working from 5 months to study the toxicity of lead acetate on PC-12 cells. So far no toxicity has been noticed even with 1000uM of lead acetate or lead nitrate. I always have participation...
08 September 2015 4,519 3 View
Does anyone have any idea why PC-12 cells after differentiation will not be die with lead exposure? I am letting them differentiated for 2 days with NGF then treat them with lead and it turned out...
04 May 2015 2,774 2 View
I want to start work with PC-12 cell line and I want to order nerve growth factor to differentiate these cells and I do not know which sort I should order. It will be so helpful if some body...
03 April 2015 3,468 6 View
I try to find thesis which have all the information about the co exposure for PCBs and MeHg
31 December 2014 7,391 0 View
is Comet assay IV have DNA% in Tail? and DNA % in head?
07 August 2014 2,009 11 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
