Is it possible to store zoospores in -80, if so, then how actually we can recover the zoospores?
Dear Aiswarya, you may be able to store P. capsici as sporangia and mycelia, using sterilezed tap water. To do this, grow first the oomicete in the most suitable agar media to produce enough sporangia, then cut discs or cubes from the culture and introduce them in a cryovial with screw cap and fill with sterilized tap water until all pieces are cover with the water. The tubes can be store in a rack at room temperature and under darkness.
Dear sir
i think you can grow and store P.capsici on Paper fruit or on Oat meal agar after that you can but some mycelium disk in glycerol .
Best regard
My colleague Aiswarya, yes you can store it but, for a period of limitation. Please, for production and store zoospores follow these steps.
1. put small cubes (0.5 -1 cm) taken from the P. capsici colony in a petri dish plate containing sterilized tap water.
2. incubate the petri dish plates under 24 h light and 22 C.
3. After 2-3 days you check the density of sporangia production.
4. Placing the plates at 4 C for 20 -30 min, and then check under a microscope to note the break of the sporangia wall and zoospores that had swum outside from it.
5. Transfering the water from plates to test tubes and collection of zoospores using weak speed centrifugal.
6. Put the zoospores in the preservation solution and store it at -80 C.
in this link, you will see the methodology
https://doi.org/10.1016/j.biocontrol.2020.104261
I think you are a person with an enterprising spirit in this domain. So I think these steps may be useful for you.
Dear Aiswarya,
Greetings and have a nice time.
Yes, you can store the sporangia of Phytophthora capsici by putting these sporangia after obtaining them by the known methods in screw vials with sterile distilled water or any other preservation solution at lab. temperature and dark condition.
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