I have DNA bands and are to be stained by SYBR Gold.
how to get links for copyrights for papers?
06 August 2024 7,410 1 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I'm new to VASP and I'm trying to calculate the band structure of a bulk, nonmagnetic superconductor with a tetragonal crystal structure. I'm including spin-orbit coupling (SOC) in my...
09 July 2024 5,641 3 View
Gender, along with socio-economic and environmental factors, is a key determinant of vulnerability to climate change.
24 June 2024 8,437 2 View
am trying to make POSCAR file and xyz file using vasp for NbReSi compound using VESTA. However, I notice that the number of atoms for xyz file is given as 41 whereas for POSCAR is it 36. Can...
17 April 2024 698 2 View
I have seen nitrogen fixation in three heterotrophs, but cannot detect any nitrogenase in their genomes. Are there any alternative pathways that I can look into that may provide information about...
01 April 2024 5,641 0 View
In XRD analysis, when we move to a higher angle, like above 50 degrees, if I have two reference lines of different phases, one matches the peak and the other is towards the right of the peak with...
31 March 2024 1,812 2 View
My study area is in eastern Himalayan foothill landscape which is a tropical to sub-tropical forest; elevation ranges from 150 to 1300 meter. The forest is some parts dominated by sal, tick and...
21 March 2024 8,297 1 View
I am trying to perform a geometry optimization (GEO_OPT) of a FCC 111 Pt_{0.5}Au_{0.5} bimetallic surface (10x10x3) generated from ASE, with a lattice parameter of 3.9 A∘A∘ in CP2K. However, after...
18 March 2024 3,328 2 View
Let me explain. I found that the structure for TiFeSi given in the ICSD database using Jsmol software is a little different from what can be obtained using Vesta software using the same cif file...
19 February 2024 9,722 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...
06 August 2024 1,350 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
