What can i do to extract antimicrobial compound from bacterial medium supernatant if do not know the nature of antimicrobial compound?

I am having an unknown bacterial hit so far, which is inhibiting the growth of multidrug-resistant bacteria. I want to characterize that antimicrobial compound.what should I do to characterize...

21 September 2020 6,869 3 View

How to identify fake research and predatory journal?

Fake researches and predatory journals

14 September 2020 4,530 7 View

For Scanning electron microscopy based trichome analysis of the leaf samples fresh samples can be preserved?

For SEM-based analysis of trichomes (soybean plant) can we preserve the samples collected for some period of time? or after following standard fixation procedures it can be preserved under low...

01 June 2020 5,665 1 View

New problem Statement on Solar Energy?

Dear All, I want to know the new research topic on Solar Energy for my PhD. work. Please let me know.

13 January 2020 4,388 1 View

What is the appropriate way / index of expressing dissimilarity of elements in a large sample collection?

Ex. I have taken a sample of x papers. Total number of contributing authors in these x papers are N authors. However, some authors have contributed in more than one papers in the sample. Say the...

03 July 2019 9,941 1 View

What is the lowest concentration you should take for determination of solubility of compounds by LC-MS method?

I have to determine solubility of LC-MS of completely new compound, however, the sample provided is very low. So, I would like to know what is the safe concentration to start.

07 February 2019 6,945 3 View

How to determine the connectivity of the pores through BET Isotherm analysis? How do we know the pores of the material are interconnected or isolated?

I have synthesized a series of porous organic polymers by tuning the linker length and observed a trend of variation of surface area (ultramicroporous, high surface area to mesoporous, low surface...

25 November 2018 3,962 2 View

Resuspend salivary proteins in 1X Coupling Buffer???

I have to enrich glycoproteins from salivary samples. I am using hydrazide resin. But it's very difficult to resuspend salivary proteins in 1X coupling buffer. Is there any solution for it?

07 September 2017 3,897 2 View

How to obtain ethical clearance for a multi-center study?

We are planning to conduct a questionnaire-based study among dental students of different dental colleges of Nepal (within a country). Should we obtain ethical clearance from each institution's...

03 October 2016 7,434 5 View

How to check titre of egg yolk antibody against bacteria?

How can I determine the titre of my egg yolk antibody against bacteria..or can anyone plz tell me how to check antibody titre against bacteria.thank you?

08 July 2016 3,224 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How can I homogenize this data to make a meta-analysis?

Hi, I am doing a review and hopefully meta-analysis for my Master thesis but I have the following problems: - Some studies only give the difference between end trial and baseline data and other...

22 February 2021 1,115 4 View

Is an issue for serial correlation when it is not found with the xtreg, fe on its lag ,but the lag become significant when you've vce(robust) std. er?

Dear Sir/Madam I have a dateset that suffers from heteroskedasticity and serial correlation. To address the serial correlation lag my variables of the fixed effect regression. I retrieve the...

15 February 2021 3,700 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

How to best represent qPCR DD Ct or D Ct as a box plot?

I've been reading up on how to best represent qPCR data, specially on answers given on this site by Dr. Jochen Wilhelm. I understand box plots are the way to go, but I still don't understand them...

11 February 2021 7,228 3 View

How do I keep duplicates based on specific variable in STATA?

Hello everybody. Im new to STATA and this forum but I hope you can help me. I am doing a research project where I intend to estimate the prevalence of venous thromboembolic events (vte) up to 30...

24 January 2021 665 5 View

Which is the correct method to show Standard Error in graphs for factorial experiment?

20 January 2021 917 2 View

Is it necessary to have internal standard in Bio analytical Method Validation in HPLC?

Please suggest a suitable reference from I can get information regarding the internal standards and other calculations for bioanalytical method validations for HPLC.

19 January 2021 5,680 5 View

At what step should one add internal standards in tissue sample for LC-MS?

Hi everyone, I'm doing metabolomics of placental tissue in my lab. I added 1ml of 80% MeOH along with 2 isotopic internal standards namely, phenylalanine and palmitic acid to my sample after...

18 January 2021 9,520 4 View

How to estimate the standard deviation of the carbom emission of a company producing many different products?

I am aware of the portfolio theory in general but want to know of a journal or author especialized in this issue.

14 January 2021 7,566 2 View