How to extract total protein from formalin-fixed animal tissue for wester blot?

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Samir Ranjan Panda

Hi Suprabhat Mukherjee

It could be very challenging due to the extensive crosslinking and fixation of the tissues. However I was able to find a few references that could help.

1. Article Protein Extraction of Formalin-fixed, Paraffin-embedded Tiss...

2. Article Efficient extraction of proteins from formalin-fixed paraffi...

3. Article An efficient procedure for protein extraction from formalin-...

Thanks