Aniket Limaye

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Questions related from Aniket Limaye

Can ethyl acetate extraction of buffered enzyme reaction prior to chiral column HPLC remove Tris salt & NADPH from the reaction sample selectively?

Hi! Using Chiral HPLC column (OB-H), I am trying to analyze final enzymatic reaction products of NADPH dependent aldo ketoreductase for possible formation of asymmetrical alcohols of...

21 August 2021 7,836 9 View

D/L or R/S chemicals, are these racemic? If I purchase such chemicals, can I obtain separate peaks for each orientation of these isomers?

Dear all, I am very new to chiral HPLC. Can someone help me understand a thing regarding chiral HPLC : I am studying stereoselective reduction of bulky ketones by recombinant aldo-keto...

16 April 2021 8,687 3 View

Why carcinoma cell lines (e.g. HepG2, Caco-2, RAW) are used in invitro cytotoxicity experiments?

HepG2 is considered as a model system for various cytotoxicity experiments according to many studies. Won't it produce wrong results if carcinoma cells have been used for such studies as such...

22 August 2017 5,427 7 View

Is there any standard criterion to select optimum number of sequence candidates for establishing a phylogeny tree in bacteria using MEGA?

for example, in case of 16s rRNA nBLAST results, I have almost 80 similar sequences with % similarity ranging from 99% and going down till 94% ?

17 March 2017 4,866 2 View