Yiannis Drosos

3 Questions 13 Answers 0 Followers

Questions related from Yiannis Drosos

Convert pLKO shRNA to mir30 based shRNA?

I want to clone shRNAs designed for pLKO.1 into the mir30 based vector pinducer10. Can I still maintain the 21-mer and short loop of the pLKO.1-based shRNA or do I need to design new shRNA with...

09 September 2016 6,056 0 View

Does anyone know which is the best way to study the activation of DNA-PK for DNA repair?

Is there a specific antibody for western blot or immunofluorescence?

08 August 2014 2,638 4 View

What is the best readout of wild type p53 activity? Is p21 always a reliable marker?

I am trying to characterize the ATM and p53 responses in a number of primary cell lines I have generated. I have been using western blot for pospho-p53 and p21 induction before and after...

08 August 2014 9,848 4 View