Is there any simple protocol for protein extraction?

More Nibras Saleam Al-Ammar's questions See All

Do you think staining the gel after electrophoresis gives good DNA visualization?

DNA visualization in the gel.

02 March 2017 8,884 8 View

Do you think visualization of DNA in gel by using dna green dye is more or less than ethBr?

Visualization DNA in the gel.

02 March 2017 7,193 5 View

If you use DNA green dye as alternative to EthBr, which color should the bands appear under U.V. light ?

I used DNA green dye as alternative to EthBr for the first time, the photos of the gel under U.V. light looks strange. this dye suppose to be green under blue light, but I have no idea about how...

02 March 2017 8,320 7 View

Do you think testing HLA matching for organ transplant with Luminex is sensitive?

Testing HLA matching for organ transplant with Luminex.

01 February 2017 2,332 4 View

How we do degasing for sephedix?

I I need to know the method used for degasing of sephedix

31 December 2016 1,140 0 View

What is the simplest protocol used for extraction and purification of extracellular proteins?

I need to extract and purify extracellular proteins.

31 December 2016 2,334 4 View

Does anyone try to amplify jak1 gene?

Do you think the most accurate method is real-time pcr?

31 December 2016 9,971 8 View

Do you think it essential to run gel after each step of protein purification?

I need to know if running gel is essential after each step.

31 December 2016 2,374 4 View

Do you think that DNA quantity and quality differs by using different protocols ?

Protocols for DNA extraction

31 December 2016 8,271 8 View

What is the priciple of Sephedix G25 in protein seperation from salts?

I want to know if this works better than other products.

31 December 2016 3,858 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Mirsada Causevic

You need to specify what your starting material is - cells or tissue. Then, what proteins are you interested in looking at - e.g. nuclear proteins, secreted proteins etc. Protein extraction protocols will depend on these characteristics.

Nibras Saleam Al-Ammar

Dear Mirsada, Thank you very much. My starting materials are T-cells. I'm interesting in extraction surface proteins of these cells for further work.