Do you think staining the gel after electrophoresis gives good DNA visualization?

DNA visualization in the gel.

02 March 2017 8,755 8 View

If you use DNA green dye as alternative to EthBr, which color should the bands appear under U.V. light ?

I used DNA green dye as alternative to EthBr for the first time, the photos of the gel under U.V. light looks strange. this dye suppose to be green under blue light, but I have no idea about how...

02 March 2017 8,193 7 View

Do you think visualization of DNA in gel by using dna green dye is more or less than ethBr?

02 March 2017 7,071 5 View

Do you think testing HLA matching for organ transplant with Luminex is sensitive?

Testing HLA matching for organ transplant with Luminex.

01 February 2017 2,210 4 View

What is the simplest protocol used for extraction and purification of extracellular proteins?

I need to extract and purify extracellular proteins.

31 December 2016 2,211 4 View

Does anyone try to amplify jak1 gene?

Do you think the most accurate method is real-time pcr?

31 December 2016 9,857 8 View

How we do degasing for sephedix?

I I need to know the method used for degasing of sephedix

31 December 2016 989 0 View

Do you think it essential to run gel after each step of protein purification?

I need to know if running gel is essential after each step.

31 December 2016 2,257 4 View

Do you think that DNA quantity and quality differs by using different protocols ?

Protocols for DNA extraction

31 December 2016 8,149 8 View

What is the priciple of Sephedix G25 in protein seperation from salts?

I want to know if this works better than other products.

31 December 2016 3,739 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View