Do you think staining the gel after electrophoresis gives good DNA visualization?

DNA visualization in the gel.

02 March 2017 8,755 8 View

If you use DNA green dye as alternative to EthBr, which color should the bands appear under U.V. light ?

I used DNA green dye as alternative to EthBr for the first time, the photos of the gel under U.V. light looks strange. this dye suppose to be green under blue light, but I have no idea about how...

02 March 2017 8,193 7 View

Do you think visualization of DNA in gel by using dna green dye is more or less than ethBr?

02 March 2017 7,071 5 View

Do you think testing HLA matching for organ transplant with Luminex is sensitive?

Testing HLA matching for organ transplant with Luminex.

01 February 2017 2,210 4 View

What is the simplest protocol used for extraction and purification of extracellular proteins?

I need to extract and purify extracellular proteins.

31 December 2016 2,211 4 View

How we do degasing for sephedix?

I I need to know the method used for degasing of sephedix

31 December 2016 989 0 View

Do you think it essential to run gel after each step of protein purification?

I need to know if running gel is essential after each step.

31 December 2016 2,257 4 View

Do you think that DNA quantity and quality differs by using different protocols ?

Protocols for DNA extraction

31 December 2016 8,149 8 View

Is there any simple protocol for protein extraction?

31 December 2016 2,246 2 View

What is the priciple of Sephedix G25 in protein seperation from salts?

I want to know if this works better than other products.

31 December 2016 3,739 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View