Do you think staining the gel after electrophoresis gives good DNA visualization?

DNA visualization in the gel.

02 March 2017 8,755 8 View

If you use DNA green dye as alternative to EthBr, which color should the bands appear under U.V. light ?

I used DNA green dye as alternative to EthBr for the first time, the photos of the gel under U.V. light looks strange. this dye suppose to be green under blue light, but I have no idea about how...

02 March 2017 8,193 7 View

Do you think visualization of DNA in gel by using dna green dye is more or less than ethBr?

02 March 2017 7,071 5 View

Do you think testing HLA matching for organ transplant with Luminex is sensitive?

Testing HLA matching for organ transplant with Luminex.

01 February 2017 2,210 4 View

What is the simplest protocol used for extraction and purification of extracellular proteins?

I need to extract and purify extracellular proteins.

31 December 2016 2,211 4 View

Does anyone try to amplify jak1 gene?

Do you think the most accurate method is real-time pcr?

31 December 2016 9,857 8 View

Do you think it essential to run gel after each step of protein purification?

I need to know if running gel is essential after each step.

31 December 2016 2,257 4 View

Do you think that DNA quantity and quality differs by using different protocols ?

Protocols for DNA extraction

31 December 2016 8,149 8 View

Is there any simple protocol for protein extraction?

31 December 2016 2,246 2 View

What is the priciple of Sephedix G25 in protein seperation from salts?

I want to know if this works better than other products.

31 December 2016 3,739 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View