Is there a scoring system available or developed by any member here for the interpretation of CXCL14 by Immunohistochemistry? Kindly share your views and insights. I am using this antibody for the first time.
After reviewing a few articles, it seems that there is no real scoring method yet. Decreased cytoplasmic expression of CXCL14 in tumor cells is believed to be correlated to bad prognosis and metastasis. I would recommend that you start by a classic H score (McCarthy). After gathering your data, you will identify the most significant cellular and/or sub-cellular compartment where CXCL14 will make sens when correlated to your clinical data on those very same tissue samples that you used to study the antibody expression.
Let me know if you need help in looking at a cohort of stained tissue, I will help you scoring them.
Sincerely,
Sihem
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I extend my gratitude Dr. Sihem for taking time to look for relevant articles, answering my question and for sharing really insightful articles for my help.
I failed to fetch any scoring system after going through few of the articles and hence was perplexed which way to design my small study of cxcl14 expression in different tumors. I wish to express more..ours is a limited facility department with no molecular facility. However it is there in other departments which can be collaborated with if need ed. At the most, we can do IHC here. I have purchased CXCL14 antibody and have just started applying on some paraffin sections.
Can you please elaborate on cohort of stained tissues please.
At last I thank you again.
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