Is there any rule for the acceptable distance between two homology regions in recombineering?

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Govinda Sharma

Couldn't you treat both adjacent regions as one very large one?

Prof. Ayad Ghany Ismaeel

Hi Colleague

Find the following URL may help you:

Article Homologous Recombination—Experimental Systems, Analysis, and...

Regards...

Aslan Massahi

Dear Govinda, it is not possible..the two regions should be at two different ends of the insert and I can not make them one, as I understand from homologous recombination

Dear Prof. Ismaeel, thank you, I will check it.