Hello,
After synthesis curcumin metal complexes, how can I purificate molecules with 1:1 and 1:2 metal-curcumin stoichiometry, and is there any reference study on this matter? In the literature, it is often mentioned that they were separated on a silica gel column, but I don't understand how they manage to do that since curcumin metal complexes strongly adhere to silica. Is this possible with chloroform or dichloromethane?
I cannot use any preparative HPLC; do you have any recommendations?
Thank you very much in advance for your responses.
I have recently synthesized several flavonoid metal complexes. I actually think that separation on silica gel is not feasible because of complex degrading. Based on my own experience, the reaction mixture (including solvent, metal salt, and ligand) needs to be crystallized over a sufficient period of time. The crystals should then (with the hope that the resulting complex is a solid insoluble in the solvent used to dissolve the ligand) should be filtered and washed with a suitable solvent to separate the ligand, residual salt.
Which metal? i have purified curcumin Ru(II) complexes on silica in my past
Metal complexes can be separated on silica gel plates. Possible eluents are ethyl methyl ketone, acetone,?, toluene thanol. These complexs jare fluorescent under UV.
Look papers by F Frya, S. Cassidy et. Al.
What about alumina and using a polar solvent like acetonitrile? I've purified metal complexes of silica with an automated flash chromatography system, and also purified metal complexes on alumina with acetonitrile as the solvent.
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