Hello,
In the literature, there are some MS/MS results that include hypothetical proteins, which can be shorter than 40 amino acids. I can also find these when I search for an organism in the protein section of NCBI. My question is, would it be absurd if I synthetically synthesize these peptides called hypothetical proteins and test them as drug candidates in certain disease models? Or are studies like the one I mentioned feasible and being conducted? If so, what procedure should I follow? For example, when I find a hypothetical protein, should I first perform a blast and then synthesize and use it if it meets certain conditions?
Is there any chance you could share some references with me that have been done in this manner?
I hope I have been able to convey what I want to ask.
Thank you for your answers.
Example link: https://www.ncbi.nlm.nih.gov/protein?term=txid562%5Borganism%3Aexp%5D+AND+((%2210%22%5BSLEN%5D+%3A+%2220%22%5BSLEN%5D)&cmd=DetailsSearch
It is hypothetical since its existence is presumed based on the transcriptomic data rather than being directly detected by proteomics or WB. You can try based on some apriori knowledge about it as a lead molecule or entirely randomly. Nothing wrong with that. Also, 'polypeptide' suits better for an amino acid chain this short.
I cannot really answer your questions, but wondered, if this will be of some help. I have seen a review article about how lactobacilli degrade milk protein, and the resulting short peptides have medicinal properties. For instance: "Many studies focused on ACE inhibitor peptides, probably
due to the ease of use of in vitro anti-ACE assays. The well-known
Val-Pro-Pro and Ile-Pro-Pro peptides are produced during milk
fermentation by some Lb. helveticus strains. ... An additional
ACE inhibitory peptide sequence (Ala-Ile-Pro-Pro-Lys-Lys-Asn-
Gln-Asp) was also identified in milk fermented by Lb. helveticus."
Raveschot, C., Cudennec, B., Coutte, F., Flahaut, C., Fremont, M., Drider, D., & Dhulster, P. (2018). Production of bioactive peptides by Lactobacillus species: from gene to application. Frontiers in Microbiology, 9, 409606.
- Badges
- Science topic
- Similar topics
- Filing