Hello,
From my (naive) understanding, there are several target genes of the canonical Wnt signaling pathway that serve as reliable/validated transcriptional readouts of pathway activity. These transcriptional pathway readouts include, among other genes: CCND1, MYC, LEF1, AXIN2.
My question is: is it possible to assess activity (activation or repression) of the non-canonical Wnt signaling pathway at the mRNA level? In other words, would I be able to quantify activity of the non-canonical Wnt signaling by, for example, performing qPCR on a set of specific non-canonical Wnt transcriptional readouts?
I have transcriptional data from an RNAseq and would like to assess whether my treatment is activating or repressing the non-canonical Wnt pathway. The problem I am running into is that transcriptional readouts of the non-canonical Wnt pathway are not specific (so they might indicate activation of the canonical Wnt pathway). Additionally, there generally does not seem to be many non-canonical Wnt transcriptional readouts from my literature searches.
I would appreciate if anyone has references or sources that would address my confusions on this topic!
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