Does the urea contain conductivity?

Solubilization buffer 2M Urea, 1mM DTT, 1mM EDTA & 10mM Tris.

31 December 2016 3,855 4 View

Is there any possibility to give negative results in ELISA? If my protein present in Urea solution

My protein present in 8M Urea, 1mM DTT, 1mM EDTA, 1mM PMSF, 10mM Nacl & 50mM Tris @pH 8.0 And it is buffer exchanged with 50mM Tris, 10mM Nacl, 1mM DTT,EDTA & PMSF @pH 8.0

31 December 2016 4,460 3 View

How can i purify my protein?

my protein PI is 5.0, with no Tag and mol.wt is 133kDa

11 December 2016 4,175 0 View

Is there any possibility of degradation of protein in 2M Urea?

My protein is recombinant one

11 December 2016 3,269 7 View

Sir/Madam please suggest me a proper protocol?

My protein is Cry1AC Mol.Wt 133kDa pI 5.0 (THEORITICAL) Protein Inclusion Bodies are forming. Please suggest me the buffers which can i use to 1. Cell Lysis (E.Coli, BL21DE3) 2. I.B Washes 3. I.B...

01 January 1970 3,471 4 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

No title

22 February 2021 9,932 11 View