my protein PI is 5.0, with no Tag and mol.wt is 133kDa
Solubilization buffer 2M Urea, 1mM DTT, 1mM EDTA & 10mM Tris.
31 December 2016 3,855 4 View
My protein present in 8M Urea, 1mM DTT, 1mM EDTA, 1mM PMSF, 10mM Nacl & 50mM Tris @pH 8.0 And it is buffer exchanged with 50mM Tris, 10mM Nacl, 1mM DTT,EDTA & PMSF @pH 8.0
31 December 2016 4,460 3 View
my protein is cry1 ac mol.wt 133.33 but it is precipitating below pH value @8.0
31 December 2016 3,490 3 View
My protein is recombinant one
11 December 2016 3,269 7 View
My protein is Cry1AC Mol.Wt 133kDa pI 5.0 (THEORITICAL) Protein Inclusion Bodies are forming. Please suggest me the buffers which can i use to 1. Cell Lysis (E.Coli, BL21DE3) 2. I.B Washes 3. I.B...
01 January 1970 3,471 4 View
I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...
22 February 2021 3,322 3 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...
17 February 2021 1,130 6 View
Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...
16 February 2021 9,050 5 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
