05 January 2020 1 8K Report

During my experiment I want to compare my PFGE band patterns with already available PFGE band patterns of CDC. But I am unable to find any such database.

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Do anyone have the R-script for analyzing POOLED data of multiple years or locations under SPLIT-PLOT design?

As we know pooled analysis is done to find out the significant different among treatment across the years/ locations. But, I am unable to analyze it for split plot design for analyzing my data. Do...

21 March 2024 1,491 2 View

Why is my western blot not transfering straight?

I have human brain tissue samples that I lyse with a nuclear extraction buffer and then the pellet I solubilized in 8M urea. I run samples in Tris-Glycine buffer in premade 4-12% gels. Ran the gel...

04 March 2024 9,722 0 View

Which media is used for culturing batrachospermales ?

I have a fresh water red algae. Which media will be suitable to grow it ?

26 February 2024 8,391 1 View

Can any one tell me Environment education related journal?

I am interested in learning about environmental science, climate change, and sustainable development concepts in children's and teachers minds and would like to do some research and review on the...

21 February 2024 2,113 3 View

Can anybidy tell me the UGC care general in Education field?

Need some quick publishing reputed name of journal for education, Science education field

07 February 2024 4,799 1 View

Why the composites exhibited a hysteresis M-H loops since the NiO is antimagnetic?

NiOCNF added with PVDF matrix , why its showing the Ferrimagnetic behaviour

15 January 2024 8,433 0 View

Missing operator Errors in VMD pathways plugin?

Hi, I'm using the VMD pathways plugin for the first time. I'm analyzing a trajectory, these are few issues I'm facing while doing it. I'll be grateful for any insight. 1. When I'm giving the...

11 December 2023 6,636 0 View

In the absorber layer of the solar cells, what is the intensity of the photoluminescence spectra?

If we use undoped materials and record their PL intensity, the intensity of the sample rises or decreases after doping. We need a low rate of recombination with a higher charge carrier...

04 December 2023 2,368 1 View

Why does my western blot gel transfer like a curved line?

I have run these westerns a billion times and something or the other doesn’t work out. For eg, my gel ran straight and fine but when I transfer the gel to my membrane, half of the membrane shows...

27 November 2023 1,494 0 View

Why does my western blot gel run like this?

I am using premade gels (4-12%) and using Running Buffer Tris Glycine Buffer and Licor SDS loading buffer

26 November 2023 8,997 0 View

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Can you explain the difference observed between my PFGE and WGS results for E. coli comparison?

Recently i did PFGE and WGS comparison with the same set of E coli strains. In my mind, if PFGE give two different pulsotypes, it is sure that strains are not the same. But when i did WGS, i...

12 May 2024 8,107 2 View

PFGE why there are no or few bands?

Hello, I am new in PFGE and started by checking Lambda ladder electrophoresis accordingly to BIO-Rad recomendations: -CHEF DR III -gel in 0.5x TBE, -recirculated at 14 °C. -Run time was 22...

18 March 2024 2,830 4 View

Confusion about virus DNA PFGE image ?

This dot appeard instead a visible distinct band. the concentration of the DNA was 30 microgeam/ml Can you help me please if this is a band or not and why it appears like that ?

10 January 2024 4,187 3 View

Does any one have any information regarding Pulse field gel electrophoresis facility available in India especially on southern parts?

Any information regarding the availability of PFGE facility in Tamilnadu?

13 June 2023 7,859 2 View

How to resolve DNA digestion problem using PFGE ?

Hi everyone, I hope someone can help me to resolve my problem. I am trying to perform PFGE on archaea (Thermococcales) but I do not understand why my genomic DNA gives a smear and not visible...

29 December 2022 2,791 6 View

Help!! How to make the PFGE of Bacillus cereus success?

I did the PFGE of Bacillus cereus many times, but there were no bands on the gel. The protocol reference is https://pubmed.ncbi.nlm.nih.gov/25862049/ What's the problem with the experiment?

04 November 2022 7,122 0 View

PFGE on Burkholderia thaliandensis?

Hi all, I am new to PFGE and I was wondering if anyone has done them specifically on B. thailandensis. I am having trouble with the ramped pulsed times. Additionally, I am not getting any bands...

11 January 2022 5,884 4 View

Does anyone have a favorite PFGE gel plug preparation method for isolating HMW DNA, and also the best agarose to use?

We are trying to isolate intact HMW DNA for our large scale genome projects. Thanks in advance.

10 November 2021 6,983 1 View

Can anybody help me to plot dendrogram?

I have PFGE data of 5 Salmonella isolates but due to technical issues I could not plot dendrogram. I appreciate if anybody could help me with this. Thanks a lot.

24 May 2021 2,201 0 View

Where in Pakistan Pulse-Field Gel Electrophoresis (PFGE) system is available?

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19 April 2021 5,386 0 View

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