Confusion about virus DNA PFGE image ?

More Mai Nasreldeen's questions See All

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,733 1 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

How to find ECC from radiation pattern on cst ?

i need to find ECC for 4-MIMO antenna using radiation pattern on CST

16 July 2024 7,291 1 View

What type of contaminant is it and how to decontaminate?

Hello everyone. I am culturing hIPSC in mTeSR-1 medium. I found some small round white particles persistently attached to cell colonies, and some actively moved under the 40X objective. Also,...

08 June 2024 6,796 5 View

Winprop feko software ?

please any one work on winprop feko software to simulate 5G antenna base station and network coverage send me LTE air interface file (.wst) that needed for network analysis

02 June 2024 1,229 0 View

Wide host range of one bacteriophage?

If one bacteriophage can infect more than a species of bacteria. this means that the phage might has 2 binding proteins binding to 2 different receptors on host cell surface? or one tail protein...

24 April 2024 9,227 4 View

Chloroform effect on bacteriophage stock?

Hello Dear Drs. During the purification of bacteriophage stock by chloroform method prior to centrifugation and filtration . Chloroform is able to lyse bacterial host cells . That means the...

25 March 2024 8,002 3 View

Western Blotting: help clearing high background when probing for AMPK / ACC pathway proteins?

This is my first post on the forum; I appreciate any help I can find here. Thank you so much! Our lab is exploring the role of the AMPK / ACC pathway in fatty liver disease. We drug-treated cells...

03 January 2024 3,891 1 View

How can use ImageJ program to separate RBC, WBC and Platelet/Fibrin from thrombus HE staining?

Hello, I need help from you guys. I have ischemia stroke thrombus HE image and I should use ImageJ (color deconvolution) to separate RBC, WBC and Platelet/Fibrin components in it as percentages...

30 November 2023 5,253 0 View

Why does size of plaques of the same phage vary from host to another that are sensitive to the phage?

Hello everyone I want to know why size or shape of plaques of same phage varies from specific host to another however the receptor is the same in all sensitive bacterial hosts and also the growth...

09 November 2023 1,115 4 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,113 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,198 4 View

What can i do to find the right topic for my thesis?

i am facing the difficulty to find the right and latest topic for my thesis. i searched and read hundreds of articles but i couldn't find the right one. what can i do I'm really confused.

02 August 2024 1,322 8 View

Hello, regarding Mxene 2D titanium carbide?

I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.

02 August 2024 6,788 4 View

Can a Conventional 50W visible LED Lamp Emit UV Light?

I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....

31 July 2024 3,842 7 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Why in 2D image created by discovery studio 2021, the halogens appear as alkyl group?

In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.

28 July 2024 734 0 View

Michael J. Benedik

I think the dot is just a water spot or a reflection of some sort and you don't have any DNA or at least not enough to see. Note you have a similar but stronger dot just to the left of the ladder band.

Mai Nasreldeen

thank you for your answer

the concentration of DNA was 30 microgram/ ml

do u think this is not enough to form a visible band ?

That is a concentration and not an amount. How much did you add?

and how do you know that concentration is accurat?