Confusion about virus DNA PFGE image ?

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Michael J. Benedik

I think the dot is just a water spot or a reflection of some sort and you don't have any DNA or at least not enough to see. Note you have a similar but stronger dot just to the left of the ladder band.

Mai Nasreldeen

thank you for your answer

the concentration of DNA was 30 microgram/ ml

do u think this is not enough to form a visible band ?

That is a concentration and not an amount. How much did you add?

and how do you know that concentration is accurat?