I have PFGE data of 5 Salmonella isolates but due to technical issues I could not plot dendrogram. I appreciate if anybody could help me with this. Thanks a lot.
a man was facing a paralysis problem.. he wants some drink or else food not even he ask, because he paralyzed .while he put the smart spectacles he blinks the eyes it will count the sensors and...
13 July 2024 8,021 1 View
The pedstrains should be counted based on the camera visuals and the data should be evaluated to turn on and off the traffic lights for the pedstrains around.
14 December 2023 8,680 5 View
Mass Transfer Coefficients: how do i calculate kla for oxygen at different agitation rates and different gas flow rates by using the Rate method and integral method? Kla Test, 1000L bioreactor,...
11 November 2023 3,475 0 View
ame across an error in PEX. In LPE .tec file, path to lvs rule file is given with Include directive.In LVS Rule file #DEFINE PEXRUN is uncommented for pexrun. Error encountered on compiling .tec...
20 September 2023 9,088 3 View
I am new to CP2K. I want a complete tutorial for the metal, which includes how to generate input files, and how to visualize the input files.
15 August 2023 2,490 0 View
I am interested in a dataset using PZT transducers for structural health monitoring of bridges.
12 August 2023 484 0 View
What could be the reason behind this? Does it mean that it is protonated?
26 June 2023 7,908 0 View
can you plese suggest other ways of restriction digestion of DNA.
19 June 2023 7,900 3 View
We performed restriction digestion and to analyze the restriction pattern by and received a bad result. What may be the reason behind it
18 June 2023 3,306 5 View
AIM: To isolate and visualize the genomic DNA from human blood sample by agarose gel electrophoresis. PRINCIPLE: Isolation of DNA usually begins with breakdown of cells. Lysed samples are mixed...
13 June 2023 8,252 0 View
Recently i did PFGE and WGS comparison with the same set of E coli strains. In my mind, if PFGE give two different pulsotypes, it is sure that strains are not the same. But when i did WGS, i...
12 May 2024 8,107 2 View
What is the breakpoint diameter for florfenicol and oxytetracycline in the disk diffusion method, indicating susceptibility or resistance to these antibiotics in the tested salmonella strain?
25 April 2024 5,168 1 View
I used these antibiotics, such as Enrofloxacin (10), Vancomycin (30), and Cefamezin (30), for Salmonella spp. as a disc diffusion method, and I'm finding it difficult to get the breakpoint for...
04 April 2024 2,710 0 View
Hello, I am new in PFGE and started by checking Lambda ladder electrophoresis accordingly to BIO-Rad recomendations: -CHEF DR III -gel in 0.5x TBE, -recirculated at 14 °C. -Run time was 22...
18 March 2024 2,830 4 View
This dot appeard instead a visible distinct band. the concentration of the DNA was 30 microgeam/ml Can you help me please if this is a band or not and why it appears like that ?
10 January 2024 4,187 3 View
Diversity, Dendrogram
05 December 2023 7,372 0 View
I would like to create a dendrogram and Jaccard similarity matrix using 0-1 gel data. However, I do not have access to NTSYS or any other specialized software. Is there an alternative method to...
23 November 2023 1,636 0 View
I see many papers that used different doses of Salmonella Enteritidis in the chicken infection model for the same type of chickens e.g., Ross-308 broiler. It also varies at different ages of the...
22 October 2023 7,725 1 View
I'm trying to calculate an MOI for an experiment between Salmonella and mammalian cells but I want to know how to calculate the amount of cells/mL in my Salmonella culture using OD600. I have done...
05 October 2023 1,761 1 View
I would like to focus on one of these foodborne pathogens in my upcoming research. E.coli O157 Salmonella Staph aureus Listeria Campylobacter But which one to start with which is more risky and...
15 September 2023 8,779 2 View