For eDNA barcoding, is there any primer published that proved to pick seaweed rbcL genes from eDNA samples? A universal primer that could amplify rbcL from Rhodophyta, chlorophyta and Ochrophyta seaweed.
Check this paper
Article DNA metabarcoding analysis of macroalgal seed banks on shell...
Thanks
Could I have the Primer sequences for ITS2, rbcl and matK for plant genetic sequence?
With all the buzz around the chatGPT and its upcoming version ChatGPT-4 which is supposed to be 100 times better, we are going to see AI generated paragraphs, articles, or even complete research...
27 January 2023 8,672 5 View
I am working on ecosystem service of forest tree species. How can I quantify the ecosystem system service of an individual tree species in a forest?
11 October 2014 9,877 11 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Reference dose and Maximum acceptable concentrations HMs
03 August 2024 8,230 4 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View