Hi guys,
Is there any new and efficient western blot apparatus that you recommend?
Trans-Blot® Turbo™ Transfer System is very fast. takes about 7 minutes to transfer proteins from gels to membrane.
I agree, Trans-Blot® Turbo™ Transfer System is very nice. You can also make your own membranes for that transfer system and not have to buy their ready membranes which can get expensive. You should also get their electrophoresis tanks for gel seperation as they also have "turbo" protocol for that.
I should also mention that you should buy their gels if you plan on doing the "turbo" protocols. They are not too expensive you can buy the material to cast it yourself rather than buying the precast ready gels. If you do not plan on using the turbo protocols, the handcast homemade polyacrylamide gels will do.
However it may be better to use the standart wet-transfer tank from bio rad if you use homemade gels as the TransBlot system is a semi-dry transfer that is optimized for bio-rad's pre-defined gels.
http://www.bio-rad.com/en-us/product/polyacrylamide-gel-reagents/tgx-fastcast-acrylamide-solutions
I agree with the people opinion above. Trans-Blot® Turbo™ works well in our lab. It is very fast as described. You do not have to buy the gels, but you need to buy the Trans-Blot® Turbo™ RTA kit. Good luck
I would also recommend the Licor Oddssey imaging system. It uses infrared secondary antibodies which are diluted to 1;30,000 to 1;40,000 which can be re-used and multiplexed, thus leading to significant cost savings in the long run. Bio Rads ChemiDoc™ MP Imaging System is also good as it can also do multiplex and has the added advantage of whole-protein normalization if you use their stain-free gels which I mentioned about previously.
- Badges
- Science method
- Similar topics
- Methodology
- Laboratory
- Laboratory Techniques
- Blotting Techniques