Is there any validated ULK2 antibody?

Hello everyone, I am recently searching an validated ULK2 antibody. However, the molecular weight by the indicated antibodies from the data sheet is around 112 kD. In my opinion, though the 112 kD...

05 June 2018 9,013 4 View

Why the efficiency of Flag antibody IP is very low?

Hi everyone, Recently, I am performing IP using anti-Flag Ab(M2, F3165) for Flag-ULK1 overexpression cells. But the efficiency is very low. Even if the input accounts for 1% of the whole cell...

07 August 2016 1,823 4 View

Which post-translation modification will change or increase the apparent electrophoresis mobility of protein?

I am recently performing Western Blot for a protein. After treatment, the apparent electrophoresis mobility increases detected by its phosphorylation antibody. Moreover, the other protein...

03 April 2016 2,576 4 View

Could a small molecule target another small molecules?

03 April 2016 4,038 5 View

No title

01 February 2016 5,020 1 View

Why do different exposure times get different results for Western blot?

11 December 2015 431 8 View

Should I boil protein samples containing GPCR or Dopamine receptors for Western Blot?

11 December 2015 2,925 11 View

Why RNAi resistant cell lines cannot rescue the phenotype?

hi everyone, I am recently performing RNAi rescue assay to validate the siRNA for gene/protein A specificity. I construct cell lines expressing wild type gene/protein A and RNAi...

10 November 2015 133 10 View

How can I get functional GPCR in cell lysate?

I am recently performing MST assay to detect the relationship between GPCR and chemical compound. I performed the MST assay using the cell lysate in HeLa cells overexpressing the GFP-tagged-GPCR...

08 September 2014 1,491 13 View

Fugene 6 transfection troubleshooting in HEK 293T

Recently I am performing the transfection assay using Fugene 6 reagent in HEK 293T cell. The plasmid includes the EGFP gene. Even I use the vector including only GFP without the fusion gene, the...

08 September 2014 4,183 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Where can I find atmospheric measurement profiles (temperature, density, etc.) between 300-800 km altitude?

I am investigating thermospheric mass density profiles between 300-800 km altitude. I would like to compare the variability with other parameters, including temperature, electron content,...

01 March 2021 5,902 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View