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Questions related from Zhiyuan li
Hi everyone, I am recently overepressing mouse ULK2 in HEK-293T cells. However, the expression level is much lower than other genes constructed with the same vector. So, is there any methods to...
06 June 2018 6,214 2 View
Hello everyone, I am recently searching an validated ULK2 antibody. However, the molecular weight by the indicated antibodies from the data sheet is around 112 kD. In my opinion, though the 112 kD...
06 June 2018 9,066 4 View
Hi everyone, Recently, I am performing IP using anti-Flag Ab(M2, F3165) for Flag-ULK1 overexpression cells. But the efficiency is very low. Even if the input accounts for 1% of the whole cell...
08 August 2016 1,935 4 View
I am recently performing Western Blot for a protein. After treatment, the apparent electrophoresis mobility increases detected by its phosphorylation antibody. Moreover, the other protein...
04 April 2016 2,661 4 View
Hi, Everyone are looking for the protein target of a small molecule, but is there any possibility that a small molecule target another small molecules? Thanks a lot in advance! Zhiyuan
04 April 2016 4,110 5 View
Hello everyone，I am recently using the coverslips （catalog：174950）from Thermanox™ Cell Culture CoverSlips. It is written "Cell Culture treatment on one side", but i cannot know which side is...
02 February 2016 5,105 1 View
Recently, I am performing Western Blot about Dopamine Receptor(DR), a GPCR (G-protein coupled receptors). And the band for DR in Western Blot was not so obvious as shown in Immunofluorescence. It...
12 December 2015 3,041 11 View
Hi everyone， 1. Our lab have two chemiluminescent substrate from Thermo Scientific (moderate) and Millipore (very sensitive). For the same blot, these two substrates get different results. 2....
12 December 2015 496 8 View
hi everyone, I am recently performing RNAi rescue assay to validate the siRNA for gene/protein A specificity. I construct cell lines expressing wild type gene/protein A and RNAi...
11 November 2015 195 10 View
Hi，guys！Does anyone know the difference between 2X and 5X sds loading buffer? It is said that maybe you cannot get appropriate results using 5X Loading buffer. Does anyone know the...
09 September 2014 6,494 14 View
I am recently using the MST (Microscale Thermophoresis) to study the interaction between the GPCR and small molecules. And I have established a stable cell line in HeLa overexpressed the...
09 September 2014 3,889 7 View
Recently I am performing the transfection assay using Fugene 6 reagent in HEK 293T cell. The plasmid includes the EGFP gene. Even I use the vector including only GFP without the fusion gene, the...
09 September 2014 4,282 7 View
I am recently performing MST assay to detect the relationship between GPCR and chemical compound. I performed the MST assay using the cell lysate in HeLa cells overexpressing the GFP-tagged-GPCR...
09 September 2014 1,562 13 View
