How to calculate the degradation rate in percentage ?

I performed the BCA assay to calculate protein content over the time period. However, I'm not understanding how I can calculate the degradation rate in percentage at each time point? What formula...

10 January 2021 9,094 3 View

Why peaks appear down in case of absorbance measurement in FTIR?

I tested the Titanium disc in FTIR after treating it with chemicals. But the absorbance peaks are showing down. I don't know what is wrong going on.

07 November 2019 1,963 5 View

I need to extract total RNA from protoplasts, could anyone help me with a detailed protocol?

22 June 2017 9,578 1 View

In the ChIP-qPCR assays what is the best method for DNA quantification and how should I normalize my data?

I started using ChIP in my analysis a short time ago and I have a lot of questions about how to normalize my data, what percentage of Input should I use and what is the best method to quantify my...

14 June 2017 7,259 3 View

Is there a model for ZF in which a topological group can fail to be completely regular?

01 June 2016 1,442 2 View

Is there any existing method, protocol or kit for gamma-glutamylcysteine synthetase assay in animal tissue?

I know about ELISA kit but we are more interested in kinetic or absorbance measurement.

08 July 2015 9,509 1 View

Does anyone have access to an H295R cell line?

I begin a project in which I have planned co-culture H295R with BeWo cell line. For actually distributed series was used Nu Serum prepared at the factory, which according to ATCC does not have the...

29 October 2014 1,080 2 View

How strongly does Hilbert projection theorem depend on CC?

01 January 1970 6,675 3 View

Research "National Identity in Food Culture on Social Media Platforms"

01 January 1970 8,420 3 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View

How to report Fishers Exact test from SPSS in a report properly?

I am wondering how do I write the data I have collected from my fishers exact test into words so that it backs up my work? the exact sig.(2-sided) was 0.033, meaning it is significant because its...

17 February 2021 3,441 7 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

How to test the equality of two proportions with multiple samplings?

02 February 2021 9,574 5 View

Western blot transfer problem - what could cause it?

Hi everyone, We are doing western blot with yeast protein extracts. We are applying a wet transfer method using Tris-Gly as a transfer buffer (with 10% methanol) and running it with constant...

25 January 2021 4,228 6 View

Is Juniper Publisher ( Journal of Gynecology and Women's Health) a good journal to publish my paper?

Dear colleagues, Do you have any knowledge about Juniper Publishers? Have any of you published in the Journal of Gynecology and Women's Health ( ISSN: 2474-7602, Impact Factor: 0.621 (2017-18))? I...

21 January 2021 9,362 4 View

I am trying the synthesis of graphene oxide by using modified hummer's method and the color of my product was white silver so is it graphene oxide?

i have tried modified hummer's method for the synthesis of graphene oxide by adding Graphite powder and NaNO3 in H2SO4 . In this mix KMnO4 was added and diluted with water . this mix was heated...

13 January 2021 9,423 4 View

Which peak should I consider when calculating ZIF-8's crystalline size from XRD?

Hello, Using the Scherrer's equation, one could calculate, roughly, the crystalline size of ZIF-8. However, I am confused as to which peak in the XRD pattern should be taken for the calculation?...

10 January 2021 8,633 3 View