Hi, here i want to ask if there is the method or solution could lyse human cells, while leave bacterial cells intact? since my samples contain both human cell and bacterial cell, and I want to collect the bacterial cell. If you know the components of this kind of solution, please send me a copy, because this is a problem for me! many thanks!
I can't think of a lysis method to do this. Are your human cells adherent? You could remove the medium to get the bacteria? Or otherwise source a filter that would let the bacteria through and retain the human cells? It's difficult to suggest without knowing what your experiment will involve.
Maybe M-PER Mammalian Protein Extraction Reagent or 1% Triton X-100. They both lyse human cells
Hi Xin,
I'd recommend using saponin, as it doesn't affect the cell walls of bacteria. With Triton- X, the common lighting agent, it could potentially do significant damage to both bacteria and mammalian cells, as it is a surfactant and not specific. Let me see if I can dig up some paperwork for you.
thats tough. many microbes are "tougher" vs human cells, and the only way to get them is bead beating or optimized enzymatic digest, in addition to chemical lysis. but still, some bacteria are very easy to lyse and will pop open along with human cells- upon very mild lysis
Runan Yan
There are some good suggestions here-
https://www.researchgate.net/post/Does-anyone-have-advice-on-lysing-eukaryotic-guts-cells-but-not-the-bacteria