17 Questions 22 Answers 0 Followers
Questions related from Xin Wang
Hi everyone, I know that TLR signal can recruit the myd88 to cell membrance from cytoplasm. Here I observed thatTLR4 protein slightly increased while total myd88 protein expression decreased. Do...
28 March 2018 2,135 2 View
Hi, Total myd88 protein contain cell membrane myd88 and cytoplasm myd88. When TLR activated, Myd88 will be recruited to the cell membrane, so how is the cytoplasm myd88? is myd88 stable in...
28 March 2018 6,529 2 View
Hello everyone, Many immune cells in mice colon can secret IFNg and IL17, for example, NK cell, CD4T and CD8 T, they all can secret the IFNg and IL17. Here I want to ask, are the IFNg or IL17...
04 February 2018 5,648 3 View
Hi everyone, I am going to use DSS to induce acute colitis, and check the immune response, including innate immune cell and adptive immune cells, here the question is that: What is the...
17 April 2017 6,319 3 View
Hi everyone, I found that mast cell protease 1 and mast cell protease 2 expression level increased 4 fold (qPCR) in colon epithelial cells of KO mice, so here I want to ask, are there any...
24 November 2016 4,973 2 View
Hi, I have some question about Peyer's patches in mice ileum: 1. What is the number of Peyer's patches in mice ileum? 2. What is the length of ileum? 10 cm? 3. I want to compare the size...
25 August 2016 9,663 1 View
Hi colleagues, Here I did microarrary analysis of the mice colon mucosa, the results shwed that Immunoglobulin heavy chain (gamma polypeptide) and immunoglobulin kappa joining 1 expression...
25 August 2016 2,970 2 View
Hi, I want to ask will collagenase digestion of mice tissue affect the certain epitopes? I use collagenase D (1 mg/ml) digest the tissue for 1 h, will this affect some epitopes? if so, how to...
08 October 2015 8,800 3 View
Hi, Here I want to analyze the T cell (from mice colon tissue), including Th1, Th17 in flow cytometry, Considering the cells number, what's the length of the colon do I need for cell collection...
28 August 2015 7,768 9 View
Hi, I need to identify four type of cells in colon tissue. 1） Macrophage 2) Monocyte 3) Neutrophils 4) Eosinophils Is there anyone who know the gating strategy (antibody selection) for these...
27 August 2015 503 6 View
Hi, Here I want to culture the mice feces bacteria, is there any medium fit for the bacteria from feces to grow? I mean how to culture the fecal bacteria as much as possible? Thanks!
20 July 2015 1,339 2 View
Hi, I want to ask what is DGGE marker used for? Since there almost no commercial DGGE marker. I can make my own marker by myself, but does the marker make any sense when you analyze the DGGE gel...
05 March 2015 4,391 10 View
Hi, here i want to ask if there is the method or solution could lyse human cells, while leave bacterial cells intact? since my samples contain both human cell and bacterial cell, and I want to...
03 March 2015 3,865 9 View
Hi, as i want to extract bacterial DNA from mice tissue, but the final solution also contains both bacterial DNA and background DNA (mice tissue DNA), Will the somatic DNA influence the bacterial...
24 February 2015 616 7 View
I am new to the DGGE primer set, I am going to research on the microbiome analysis through DGGE, but I would like to ask is there any DGGE primer set fit for universal bacteria? And is there any...
20 February 2015 9,403 2 View
Hi, Can NGS analysis with 16S rRNA gene sequencing get microbiome information of both Bacteria and Fungus with same primer set?? I mean when you do microbiome analysis(soil or feces), how do you...
20 February 2015 4,387 5 View
Mice colon has many different immune cells, I need to isolate the neutrophils, macrophages from colon, mucosa, does any one know the protocol regarding this method? Great thanks!
27 January 2015 5,077 2 View
