Xin Wang

13 Questions 16 Answers 0 Followers

Questions related from Xin Wang

MyD88 protein is membrane protein or cytoplasm protein ?

Hi everyone, I know that TLR signal can recruit the myd88 to cell membrance from cytoplasm. Here I observed thatTLR4 protein slightly increased while total myd88 protein expression decreased. Do...

28 March 2018 2,121 2 View

What is the signal for specific secretion of IFNg/IL17 by different immune cells ?

Hello everyone, Many immune cells in mice colon can secret IFNg and IL17, for example, NK cell, CD4T and CD8 T, they all can secret the IFNg and IL17. Here I want to ask, are the IFNg or IL17...

04 February 2018 5,638 3 View

What is the best timepoint to ckeck the immune cells for DSS-induced acute colitis?

Hi everyone, I am going to use DSS to induce acute colitis, and check the immune response, including innate immune cell and adptive immune cells, here the question is that: What is the...

17 April 2017 6,308 3 View

Anyone knows about mast cell activation signal pathway?

Hi everyone, I found that mast cell protease 1 and mast cell protease 2 expression level increased 4 fold (qPCR) in colon epithelial cells of KO mice, so here I want to ask, are there any...

24 November 2016 4,965 2 View

What is the number of Peyer's patches in mice ileum?

Hi, I have some question about Peyer's patches in mice ileum: 1. What is the number of Peyer's patches in mice ileum? 2. What is the length of ileum? 10 cm? 3. I want to compare the size...

25 August 2016 9,653 1 View

What is Immunoglobulin heavy chain (gamma polypeptide)? belong to IgA or IgG?

Hi colleagues, Here I did microarrary analysis of the mice colon mucosa, the results shwed that Immunoglobulin heavy chain (gamma polypeptide) and immunoglobulin kappa joining 1 expression...

25 August 2016 2,962 2 View

Will collagenase digestion of mice tissue affect the certain epitopes like CD45 ?

Hi, I want to ask will collagenase digestion of mice tissue affect the certain epitopes? I use collagenase D (1 mg/ml) digest the tissue for 1 h, will this affect some epitopes? if so, how to...

08 October 2015 8,793 3 View

How much colon tissue is needed for T cell gating by flow cytometry?

Hi, Here I want to analyze the T cell (from mice colon tissue), including Th1, Th17 in flow cytometry, Considering the cells number, what's the length of the colon do I need for cell collection...

28 August 2015 7,760 9 View

Which antibody can I use for macrophages, monocytes, neutrophils and eosinophils in mice colon?

Hi, I need to identify four type of cells in colon tissue. 1） Macrophage 2) Monocyte 3) Neutrophils 4) Eosinophils Is there anyone who know the gating strategy (antibody selection) for these...

27 August 2015 495 6 View

What is DGGE marker used for ?

Hi, I want to ask what is DGGE marker used for? Since there almost no commercial DGGE marker. I can make my own marker by myself, but does the marker make any sense when you analyze the DGGE gel...

05 March 2015 4,385 10 View

Will somatic DNA influence the bacterial DNA when doing downstream PCR analysis?

Hi, as i want to extract bacterial DNA from mice tissue, but the final solution also contains both bacterial DNA and background DNA (mice tissue DNA), Will the somatic DNA influence the bacterial...

24 February 2015 607 7 View

Is there any DGGE primer set for universal bacteria and special type?

I am new to the DGGE primer set, I am going to research on the microbiome analysis through DGGE, but I would like to ask is there any DGGE primer set fit for universal bacteria? And is there any...

20 February 2015 9,396 2 View

How to isolate different immune cells like neutrophils in mice colon?

Mice colon has many different immune cells, I need to isolate the neutrophils, macrophages from colon, mucosa, does any one know the protocol regarding this method? Great thanks!

27 January 2015 5,071 2 View