Dear all, in order to perform life calcium imaging experiments I would like to use commercial isotonic solutions available in the market instead to prepare them by myself.
I believed that HANKS' balanced salt solution (HBSS) was an option. However I cannot find the same solution just with or without calcium, without affecting the Mg content. I find Ca and Mg are always coupled in HBSS.
Check LifeTechnologies as example: https://www.lifetechnologies.com/nl/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/balanced-salt-solutions/hbss-hanks-balanced-salt-solution.html
Please, any suggestion?
Go to SIGMA, they have hundereds of solutions with and without calcium.
Calcium free is for example Joklik modification Minimum Essential Medium (MEM) Eagle, and the plain MEM has calcium. Depending on your cell type you can pick from hundreds of solutions.
Just add the required amount of MgCl2 in the commercially available HBSS ca- solution.
I will go for the HBSS without Ca+Mg. I will add the right amount of Mg. So, starting with the same solution, in case of Ca ions are needed, I will add them. To get solutions of the same osmolarity, I will add NaCl to compensate the final osmolarity. The total amount of NaCl will be negligible. Thanks both, Tamas and Manoj.
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