Ivan Lorenzo Moldero

3 Questions 5 Answers 0 Followers

Questions related from Ivan Lorenzo Moldero

Any opinion on the formulation of the isotonic solution in live calcium imaging?

Hi, we culture human MSC in alpha-MEM+10%FBS and the basic media contains 0.8mM MgSO4, 1.8mM CaCl2, 5.5mM Glucose, and 117mM NaCl. I want to prepare a solution containing 0.8 mM MgCl2, 1.8mM...

14 March 2016 6,080 3 View

Is there any kind of commercial isotonic solution -with and without calcium and the same osmolarity- to carry out life calcium experiments?

Dear all, in order to perform life calcium imaging experiments I would like to use commercial isotonic solutions available in the market instead to prepare them by myself. I believed that HANKS'...

23 April 2015 6,783 3 View

What is the suitable calcium indicator for nuclear calcium imaging?

I want to carry out calcium signaling experiments in MSC cultured in 3D scaffolds.Due to equipment constrains, I only can exite the fluorocrome probe above 450nm. So, I am considering to use...

31 March 2015 4,647 8 View