I tried to isolate Genomic DNA from Jatropha curcas with high yield manually (CTAB method), but did not succeed. I tried genomic DNA isolation kit from HiMedia, Genetix . Please, can you suggest to me a DNA isolation kit for Jatropha curcas.
cDNA sequencing
Total RNA was extracted from leaf and callus tissue using an RNeasy Plant Mini Kit (Qiagen, Germany). mRNA was purified from the total RNA using Oligotex-dT30 (Takara Bio Inc., Japan). Sequencing was performed with a GS FLX Instrument (Roche Diagnostics) using the cDNA rapid library method according to the manufacturer's instructions.
Ref:
1) Sequence Analysis of the Genome of an Oil-Bearing Tree, Jatropha curcas L. DNA Res. Feb 2011; 18(1): 65–76.
2) An improved method for RNA isolation and cDNA library construction from immature seeds of Jatropha curcas L
Jatinder Singh Sangha12*, Keyu Gu1, Jatinder Kaur3 and Zhongchao Yin1
BMC Research Notes 2010, 3:126 doi:10.1186/1756-0500-3-126
The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1756-0500/3/126
Hi, this kit works well:
http://www.mn-net.com/Products/NucleicAcidPurification/DNAfromplantandfungi/NucleoSpinPlantII/tabid/1484/language/en-US/Default.aspx
good luck
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