I am working on the capillary formation of Lymphatic endothelial cells in a collagen matrix. But I am facing a technical problem to handle Collagen. I have made many times; I am not able to remove the bubbles from the collagen. After 2 hours of gel solidification of collagen gel, It is showing dark color in place transparent matrix.
If anyone gives suggestions to resolve this technical problem, I will be grateful.
Dear Devendra!
Please You look at this article:
Article Preparation of 3D Collagen Gels and Microchannels for the St...
Protocol
1. Neutralization, Dilution and Polymerization of Collagen Solutions for 3D Investigation and Cellular Contraction Assays
3. Generation of PDMS Microchannels for Collagen Fiber Alignment
We alway centrifuged our native collagen solutions in the cold before polymerization and our dissolved gelatin solutions hot before gelling. The bubbles rise to the surface and can be wiped off with sterile filter paper or pipetted off.
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