I would appreciate it very much if RG members could suggest any good and (preferably GPL) alternative to GeneMapper and Geneious software for microsatellite (SSR) analysis. Thanks.
I used PeakScanner for allele scoring and followed by GenAlex, Arlequin, or some R packages for downstream analysis.
For allele scoring I use STRand https://vgl.ucdavis.edu/STRand
It is pretty convenient except for data storage which is a bit cumbersome.
For analysis (assuming population genetics) I can recommend GenAlEx https://biology-assets.anu.edu.au/GenAlEx/Welcome.html
Runs on Excel, allows for a pretty good range of analysis itself and automatic export for a lot of formats for other software (i.e. STRUCTURE)
You can use Mapmaker too for the analysis
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