Calculate the spore count using the equation: spores/ml = (n) x 10^4, where: n = the average cell count per square of the four corner squares counted

Majid Jabir whats that 10^4 ?

It depends on the calibration of the muller counter you should check its manual

Excellent method for determining numbers of P. infestans sporangia, zoospores or spore number of other fungi.

1. Prepare a spore suspension.

2. Prepare a hemacytometer for use.

a. Carefully clean all surfaces of the hemacytometer and cover-slip.

b. Take care to ensure that all surfaces are completely dry using non-linting tissue.

c. Center the coverslip on the hemacytometer.

3. Pipet approximately 9 microliters (this volume will vary slightly with the brand of hemacytometer) of the cell suspension into one of the two counting chambers.

a. Use a clean pipet tip.

b. Be sure that the suspension is thoroughly, but gently, mixed before drawing the samples.

c. Fill the chambers slowly and steadily.

d. Avoid injecting bubbles into the chambers.

e. Do not overfill or underfill the chambers.

4. Count the spores.

b. Count all of the spores in each of the four 0.1 mm³ corner squares.

1) DO count the spores touching the top or left borders.

2) DO NOT count the spores touching the bottom or right borders.

Determine the Spore Count.

a. Calculate the total spores counted in the four corner squares.

b. Calculate the spore count using the equation: spores/ml = (n) x 10^4, where: n = the average cell count per square of the four corner squares counted.

Example: If the calculated average (n) of spores in the four 1 mm corner squares of the hemacytometer is 30:

cells/ml = (n) x 10^4 OR spores /ml = 30 x 10,000 = 300,000 spores /ml.

Thanks to Julious Pyton.....

Michael Dare Asemoloye what is this calibration of the muller counter ?

Priya Dharshana whats that 10^4 ?

The equation formula is depend on which haemacytometee that you use. It has size and depth spesifically so please check your manual instruction.

and best is using 5 squares area not only 4 squares in the corner, add 1 more square in the center/middle! And if the differences between squares is more than 20 cells, please repeat again by homogenize your cell suspension. The diffefences should not be more than 20 cells between counted square (to indicate your cell suspension is completely homogenized)

goodluck

Wiwiek Harsonowati i am using haemacytometer with 4 squares area ,

so which formula?

Spore/ml : average amount of spores from 4 squares x Dilution factor x 1000

1000 is from uL spore suspension to convert to mL

Wiwiek Harsonowati Thanks , i got it

Dr- Hafiz Muhammad Rizwan Thanks Allah finally you got it 🤣🤣

Ashwani Kumari Ashwani Kumari Thank you very much for sharing