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Questions related from Dr- Hafiz Muhammad Rizwan
Dear Researchers, For the molecular identification of fungus, why usually use the three regions internal transcribed spacers (ITS), Small subunit (SSU), and large subunit (LSU)?
11 October 2021 6,450 4 View
Dear researchers, I used Agrobacterium tumefaciens to transform Arabidopsis thaliana, and the vectors were constructed on pCAMBIA1301 and pCAMBIA1302 with a hygromycin resistance gene on them. I...
22 August 2021 1,239 26 View
Dear Researchers, 1- Is the reporter gene, such as pCAMBIA1301 with GUS and pCAMBIA1301 with GFP, affecting the transformation efficiency? 2- Is the vector length, such as pCAMBIA1301 (11,850 bp)...
05 July 2021 8,047 5 View
Dear researchers, The attached images are from the leaves of transformed plants by empty vector pCAMBIA1302 (GFP), so the images are ok and can use for a manuscript? Your comments are...
15 April 2021 6,009 11 View
Standard software such as GeNorm (qbase +), Normfinder, BestKeeper and RefFinder cannot be accessed for free. If you have a link for easy access, please share the software's/links, or if you have...
20 April 2020 8,311 0 View
I am using LightCycler® 96 for absolute quantification, and want to generate a standard curve, as I am using one "Reference gene" with the 10 different samples with different cDNA dilutions such...
26 February 2020 1,791 5 View
I have a different type of fungal colonies, isolated from fruit post-harvest and now I want to calculate their spore concentration so: 1-Is there any general formula for fungal spore...
06 January 2020 5,173 12 View
Hi, As we know that in vitro regeneration depends on different factors but my main focus is on macro nutrients especially Nitrogen level in MS media with combination of different PGRs. i...
05 January 2018 4,505 7 View
Dear Researchers, I did GC-MS by using the Agilent Masshunter and the file are in .D format, the Masshunter software is available in the GC-MS lab,(which is not in access all the time) but...
01 January 1970 6,410 0 View