Dr- Hafiz Muhammad Rizwan

9 Questions 42 Answers 0 Followers

Questions related from Dr- Hafiz Muhammad Rizwan

Why the three regions (ITS,SSU and LSU) used to identify fungal pathogens?

Dear Researchers, For the molecular identification of fungus, why usually use the three regions internal transcribed spacers (ITS), Small subunit (SSU), and large subunit (LSU)?

11 October 2021 6,453 4 View

Why do Arabidopsis seedlings transformed on MS+ Hygromycin (50mg/L) medium look the same as the negative control (col-0)?

Dear researchers, I used Agrobacterium tumefaciens to transform Arabidopsis thaliana, and the vectors were constructed on pCAMBIA1301 and pCAMBIA1302 with a hygromycin resistance gene on them. I...

22 August 2021 1,243 26 View

Is the reporter gene and vector length, such as pCAMBIA1301 with GUS and pCAMBIA1301 with GFP, affecting the transformation efficiency?

Dear Researchers, 1- Is the reporter gene, such as pCAMBIA1301 with GUS and pCAMBIA1301 with GFP, affecting the transformation efficiency? 2- Is the vector length, such as pCAMBIA1301 (11,850 bp)...

05 July 2021 8,048 5 View

What about the following GFP images, form the leaves of transformed plants by empty vector? ?

Dear researchers, The attached images are from the leaves of transformed plants by empty vector pCAMBIA1302 (GFP), so the images are ok and can use for a manuscript? Your comments are...

15 April 2021 6,010 11 View

Can someone share the software's and supported files (qbase+, NormFinder, BestKeeper and RefFinder) for qPCR experiments?

Standard software such as GeNorm (qbase +), Normfinder, BestKeeper and RefFinder cannot be accessed for free. If you have a link for easy access, please share the software's/links, or if you have...

20 April 2020 8,312 0 View

How to generate qPCR standard curve in LightCycler® 96?

I am using LightCycler® 96 for absolute quantification, and want to generate a standard curve, as I am using one "Reference gene" with the 10 different samples with different cDNA dilutions such...

26 February 2020 1,792 5 View

Is there any general formula for fungal spore calculation using Hemocytometer?

I have a different type of fungal colonies, isolated from fruit post-harvest and now I want to calculate their spore concentration so: 1-Is there any general formula for fungal spore...

06 January 2020 5,174 12 View

In vitro Effect of macro nutrients on embryogenci callus induction and shoot multiplication?

Hi, As we know that in vitro regeneration depends on different factors but my main focus is on macro nutrients especially Nitrogen level in MS media with combination of different PGRs. i...

05 January 2018 4,506 7 View

How can convert .D file to AIA/MSD file format?

Dear Researchers, I did GC-MS by using the Agilent Masshunter and the file are in .D format, the Masshunter software is available in the GC-MS lab,(which is not in access all the time) but...

01 January 1970 6,411 0 View