In Sanger sequence, after having sequencer output (ab1 file), I would like to apply a free software to check the mutation on level of transcript. I was already working with “mutation Surveyor” software, but it is not freeL. Is there any free software? Thanks a lot.
There is also another possibility you can give a try. May be it serves your purpose.
http://www.mutationtaster.org/
Thanks Abhijeet, mutationtaster is good but I want to put ab1 file(sequencer output) in the software and get the mutation in cDNA level for example c.1257A>G. Mutation Taster cannot do this.
Thanks Abhijeet,
UniproUgene sounds promising in this case. when I go to Tools>Sanger data analysis>Map reads to reference
I face with this sentence:
Paths for the following tools are not selected: BlastN, make BlastDB.
I have problem in this step and cannot go ahead.
Do you have any idea or tutorial video to help me.
Thanks Abhijeet,
After selecting Blast all path, I tried to use Sanger data analysis>Map reads to references
I uploaded reference file and also sequencer output (ab1 file) in "read" section. But I faced with this error:
[ERROR][22:00] process:3464>Subtask {Compose alignment} is failed: No read satisfy minimum similarity criteria.
I attached screenshot from the message and also enclosed reference file + read file(ab1 file).
Could you please kindly check it by your computer and see what is wrong?
Thanks in advance for your kind cooperation.
Sincerely
Omid
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