Hi all,
I am planning to study neurosphere and neural stem cells and as per protocol I am supposed to use fgf2 as a growth supplement. Since I am new to this I am trying to find out some cost effective alternate to expensive chemicals so that i can perform and optimize my study with minimum requirements and cost.
Also I will be happy if someone working on neural stem cells and neurosphere can share there protocol for the study or can guide me with critical factors of the culture which should be taken care off.
I have sorted out a research article (attached) which I am planning to follow for my study, please do share your views on the protocol attached.
Any other suggestions are most welcome
Thank you
Shubham Dwivedi
All I will say is try to shop around. The vendor used by my laboratory was PeproTech; they sold recombinant FGF2. Some vendors give discounts if buying more (e.g. 3-for-2) or will discount if they become a preferred vendor at your institution. India has many new biotech companies; try ordering in region because of shipping on dry ice and integrity of the product. Hope this helps.
Regards,
Robbie Singh, MS, MBA
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