Shubham Dwivedi

8 Questions 15 Answers 0 Followers

Questions related from Shubham Dwivedi

What if BBB is showing Excellent for a molecule in ADMETlab 2.0?

Hi all, I am looking for drugs targeting neurological disorders and thus should cross the blood-brain barrier (BBB). However, when I run the molecule on ADMETlab 2.0, it shows Red (++) for BBB...

04 August 2023 3,192 0 View

Concentration and time of exposure of Glutamate to be used for screening molecules against glutamate induced exocitotoxicity ?

Dear All, I am want to screen my compounds for rescue against Glutamate induced excocitotoxicity on rat primary cortical neurons. I referred few papers suggesting glutamate exposure for 24 hrs...

18 October 2018 9,793 0 View

Which one is better and why: Microarray or Transcriptome Analysis

Dear all, We have developed a model on autism using zebrafish. Keeping this model in mind we are looking forward to explore the probable pathways involved in model. As per the literature I found...

18 July 2017 1,071 3 View

Is there any economical alternative to basic fibroblast growth factor (fgf2) which can be used to culture neurosphere and neural stem cells?

Hi all, I am planning to study neurosphere and neural stem cells and as per protocol I am supposed to use fgf2 as a growth supplement. Since I am new to this I am trying to find out some cost...

12 May 2017 6,751 1 View

How to do ROS studies and MTT Assay with SHSY-5Y Cell lines?

Hi All.. I am trying to do ROS as well as MTT assay with SHSY-5Y cell lines. I have tried to check LPS induced cytotoxicity on cells using MTT, where I plated 6-7 x 103 cells per well and treated...

18 March 2016 1,335 9 View

Any cost effective alternative to MPTP which can be used to induce Parkinson (repeatably) in rodents?

I am looking for an cost effective chemical which can be used as an alternative to MPTP in parkinson model of rodents. Since I have to study many compounds time to time so I need to go for some...

18 June 2015 6,431 4 View

Can Anti-GFP antibody used for labelling YFP as well?

Hello everyone, I am looking for antibody against EYFP. I have searched on Sigma, Thermoscientific, Abcam, and Cell signaling technology for EYFP antibodies. However, the search results show...

01 January 1970 3,778 8 View

Can we use differentiated IMR-32 cells for Glutamate excocitotoxicity assay ?

Dear All, I am trying to establish a screening method for glutamate receptor antagonist (mGluR5) for preliminary screening. Most of literature suggest use of primary neuronal culture for the...

01 January 1970 2,795 0 View