Why topoisomerase (I and II) assays are performed using drug concentration of 50 or 100 micro-molars?

I have consulted multiple publication where Topoisomerase assays are performed using drug concentration of 50 and 100 micromolar? why such high concentration used for this assay? Is there any...

05 June 2019 5,962 1 View

What is most reliable method for testing the antileishmanial activity induced by small molecule inhibitors in vitro using promastigotes culture?

The literature reveals array of assay including assay using tetrazolium dyes, using mix of sybr green in Triton, counting using cytometer, etc. I would appreciate if the expert working in said...

04 May 2019 3,148 3 View

No title

I am bit confused with how exactly to write the results in terms of molarity for the said statement (1 pmol = 1.0E-12 mol). Any suggestions and help would be really appreciable.

31 December 2018 8,290 1 View

HRMS data showing mass less than 16 amu from the exact mass?

Hi, I am analysing my HRMS spectral data, and I observed my mass to be 457.1980, which is having 16 amu difference from the calculated mass 473.2216 (Chemical Formula: C30H27N5O). I have duly...

05 June 2018 6,433 21 View

Is compensation done in flow cytometry can be applied to single sample or is it mandatory to apply the same compensation to all samples under study?

I am using two dyes in flow cytometer and they spill over in some of the samples, so should i apply compensation to only those individual samples having spill over or it is mandatory to apply the...

03 April 2018 3,997 2 View

How to synthesize N-formyl containing molecules using deuterated formic acid (DCOOH)?

I have been synthesizing N-formylated compounds previously using formic acid, but when the formic acid was replaced with deuterated formic acid reaction is not progressing under similar...

02 March 2018 1,507 5 View

What will the effect of Xanthine Oxidase Inhibition on HIF-alpha gene expression?

In my experiment i am getting increased expression of HIF-1alpha gene after the qPCR experiments. the results are consistent with allopurinol and other synthetic molecule. Kindly help me with...

11 December 2017 7,101 2 View

Is it necessary that VEGF gene expression decreases every time with anticancer drug treatment?

VEGF has been related to angiogenesis in cancer and it expression is reported to decrease after treatment with a potent anticancer agents. In my experiment (gene expression) i am failing to get...

10 November 2017 1,595 7 View

What are the various in vitro and in silico studies need to be done for the assessment of toxicity profile of a synthetic molecule?

Any research papers, protocols based on mentioned theme will be helpful.

10 November 2016 4,287 0 View

Which is the best fixing solvent or reagent used to increase permeability of nuclear membrane for Propidium iodide?

31 December 2015 8,009 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View