Gaurav Joshi

14 Questions 21 Answers 0 Followers

Questions related from Gaurav Joshi

Why topoisomerase (I and II) assays are performed using drug concentration of 50 or 100 micro-molars?

I have consulted multiple publication where Topoisomerase assays are performed using drug concentration of 50 and 100 micromolar? why such high concentration used for this assay? Is there any...

06 June 2019 6,036 1 View

What is most reliable method for testing the antileishmanial activity induced by small molecule inhibitors in vitro using promastigotes culture?

The literature reveals array of assay including assay using tetrazolium dyes, using mix of sybr green in Triton, counting using cytometer, etc. I would appreciate if the expert working in said...

05 May 2019 3,250 3 View

How to condense the following statement in terms of molarity: "10 picomoles of H2O2 in a 100 µL volume"?

I am bit confused with how exactly to write the results in terms of molarity for the said statement (1 pmol = 1.0E-12 mol). Any suggestions and help would be really appreciable.

01 January 2019 8,364 1 View

HRMS data showing mass less than 16 amu from the exact mass?

Hi, I am analysing my HRMS spectral data, and I observed my mass to be 457.1980, which is having 16 amu difference from the calculated mass 473.2216 (Chemical Formula: C30H27N5O). I have duly...

06 June 2018 6,555 21 View

Is compensation done in flow cytometry can be applied to single sample or is it mandatory to apply the same compensation to all samples under study?

I am using two dyes in flow cytometer and they spill over in some of the samples, so should i apply compensation to only those individual samples having spill over or it is mandatory to apply the...

04 April 2018 4,075 2 View

How to synthesize N-formyl containing molecules using deuterated formic acid (DCOOH)?

I have been synthesizing N-formylated compounds previously using formic acid, but when the formic acid was replaced with deuterated formic acid reaction is not progressing under similar...

03 March 2018 1,592 5 View

What will the effect of Xanthine Oxidase Inhibition on HIF-alpha gene expression?

In my experiment i am getting increased expression of HIF-1alpha gene after the qPCR experiments. the results are consistent with allopurinol and other synthetic molecule. Kindly help me with...

12 December 2017 7,206 2 View

Is it necessary that VEGF gene expression decreases every time with anticancer drug treatment?

VEGF has been related to angiogenesis in cancer and it expression is reported to decrease after treatment with a potent anticancer agents. In my experiment (gene expression) i am failing to get...

11 November 2017 1,678 7 View

What are the various in vitro and in silico studies need to be done for the assessment of toxicity profile of a synthetic molecule?

Any research papers, protocols based on mentioned theme will be helpful.

11 November 2016 4,370 0 View

Which is the best fixing solvent or reagent used to increase permeability of nuclear membrane for Propidium iodide?

Actually, in my experiment, I was using 70% ethanol up till now as a fixing solvent for increasing the permeability of the nuclear membrane. Also, I have read about paraformaldehyde and some...

01 January 2016 8,087 3 View

Is there any correlation between EGFR and HDAC on receptor basis?

A drug currently developed to target EGFR-HDAC, CUDC-101 is in phase -1 trial and claims to show dual inhibitory activity on both EGFR and HDAC

08 August 2014 1,084 2 View

How to completely remove iron (Fe) powder from the reaction mixture?

I am basically using a mixture of iron and hydrochloride to reduce an aromatic nitro group into an amine, but the problem is that the iron is still left in the reaction mixture. I have tried...

05 May 2014 3,929 7 View

Is it possible to synthesize diamond in near future?

Diamond is a metastable allotrope of carbon, where the carbon atoms are arranged in a variation of the face-centered cubic crystal structure called a diamond lattice.

01 January 2014 7,269 2 View

If I synthesize pyrazolines from chalcones using hydrazine hydrate in acetic acid medium, will I definitely get N-acetylated pyrazolines?

I just want to synthesize simple pyrazolines with no N-acetylation. Is the acetic acid method reliable to do so, or is there a preferable method to get pyrazolines other than using methanol as...

12 December 2013 6,017 5 View