Why topoisomerase (I and II) assays are performed using drug concentration of 50 or 100 micro-molars?

I have consulted multiple publication where Topoisomerase assays are performed using drug concentration of 50 and 100 micromolar? why such high concentration used for this assay? Is there any...

05 June 2019 5,962 1 View

What is most reliable method for testing the antileishmanial activity induced by small molecule inhibitors in vitro using promastigotes culture?

The literature reveals array of assay including assay using tetrazolium dyes, using mix of sybr green in Triton, counting using cytometer, etc. I would appreciate if the expert working in said...

04 May 2019 3,148 3 View

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I am bit confused with how exactly to write the results in terms of molarity for the said statement (1 pmol = 1.0E-12 mol). Any suggestions and help would be really appreciable.

31 December 2018 8,290 1 View

HRMS data showing mass less than 16 amu from the exact mass?

Hi, I am analysing my HRMS spectral data, and I observed my mass to be 457.1980, which is having 16 amu difference from the calculated mass 473.2216 (Chemical Formula: C30H27N5O). I have duly...

05 June 2018 6,433 21 View

Is compensation done in flow cytometry can be applied to single sample or is it mandatory to apply the same compensation to all samples under study?

I am using two dyes in flow cytometer and they spill over in some of the samples, so should i apply compensation to only those individual samples having spill over or it is mandatory to apply the...

03 April 2018 3,997 2 View

How to synthesize N-formyl containing molecules using deuterated formic acid (DCOOH)?

I have been synthesizing N-formylated compounds previously using formic acid, but when the formic acid was replaced with deuterated formic acid reaction is not progressing under similar...

02 March 2018 1,507 5 View

What will the effect of Xanthine Oxidase Inhibition on HIF-alpha gene expression?

In my experiment i am getting increased expression of HIF-1alpha gene after the qPCR experiments. the results are consistent with allopurinol and other synthetic molecule. Kindly help me with...

11 December 2017 7,101 2 View

Is it necessary that VEGF gene expression decreases every time with anticancer drug treatment?

VEGF has been related to angiogenesis in cancer and it expression is reported to decrease after treatment with a potent anticancer agents. In my experiment (gene expression) i am failing to get...

10 November 2017 1,595 7 View

Which is the best fixing solvent or reagent used to increase permeability of nuclear membrane for Propidium iodide?

31 December 2015 8,009 3 View

Is there any correlation between EGFR and HDAC on receptor basis?

A drug currently developed to target EGFR-HDAC, CUDC-101 is in phase -1 trial and claims to show dual inhibitory activity on both EGFR and HDAC

07 August 2014 1,016 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View